Qubes_dom0-root and other key files don't exist error after trying to EFI partition

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Did you configure sys-usb for your Qubes last time when you booted it?
Make a photo of module2 line like in this screenshot:

1920×1080 56.5 KB

By following steps from here:

Press the E key on the first prompt (or your custom prompt). Then, press the down arrow key multiple times to reach the line starting with module2.

Autostart troubleshooting | Qubes OS

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