Yubikey is not working with Qubes OS 4.1.0

I am testing Qubes OS 4.1.0, which is installed on a USB-Stick. However my Yubikey 5 NFC, which I use since 2019 with Qubes OS 4.0.4 is not working. When I try to use it for example with my Google account. The Yubikey is simply not shown in the USB-manager, like it used to work with Qubes OS 4.0.4.
Please help me.

Thank you very much.

Stay healthy & safe.

Best Knut by Walter

Hello, this may be helpful: U2F proxy | Qubes OS

Another approach, if you have a sys-usb qube, could be to attach the yubikey block device directly to the qube where you’re trying to login from (though this might have security implications I don’t yet understand, so you should do your own research or wait for more experienced feedback)

You can only see it in the USB manager if you have sys-usb running.

U just installed uqubes-u2f-proxy and for me it’s also not work like guideline says. Maybe we should try if there is on change in the policy that enforcing passing only secrets enrolled in qube?

No, It just not working. Maybe It is worth creating an issue?

Did you assign the yubikey to the VM running the browser?

Yes. I found the root of the problem. I have no qubes-u2f-proxy enabled in sys-usb which according to guide should be auto enabled by default, and only when you have other usb qube you should change this setting.