same here with me. I solved it by uninstalling pipewire in dom0:
sudo dnf remove pipewire
as qubes_lover pointed out here:
I did not have to do any modifications in templates however.
It would be interesting if this problem also occurs after a clean 4.2. install
(obsolete: before that I always had to do my workaround:
Settings–> pulse Audio Volume there I have to change the profile (which is set to analog audio output) to something different and than back to analog audio output. )