Maybe your second OS overwritten your Qubes OS grub config.
I don’t have PC with legacy BIOS to test it but you can try to restore your Qubes OS grub config by decrypting Qubes OS dom0 root, mounting, chrooting into it and then running grub2-mkconfig there.
You can mount and chroot into dom0 root like this (this guide is for EFI so you’ll have to adapt it for legacy BIOS):