Qubes external SSD

If you’re booting in EFI mode then try to add the Qubes OS EFI boot entry manually:

But I think it’ll disappear again when you disconnect your external SSD from your PC and boot it without it connected.
In Qubes OS 4.2 this issue was fixed:

And you no longer have to manually create a fallback EFI path but if you’ve upgraded in-place then maybe you’ve missed this fix and you need to manually add the fallback EFI path as described in the issue.