How to change boot options for USB boot

If you meant the “Qubes 4.0: EFI” way, then it’s only for old Qubes OS 4.0 version, not for newer Qubes OS versions.
For Qubes OS 4.2 and newer you need to edit the default GRUB config /etc/default/grub and regenerate the GRUB config using grub2-mkconfig -o /boot/grub2/grub.cfg command for both Legacy BIOS and UEFI.

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