Building Qubes .iso?

Did you try latest Qubes ISO build with kernel-latest from https://qubes.notset.fr or https://openqa.qubes-os.org?
Does it work fine for you?
Also if you have problems with kernel-latest-5.16.11-1.fc32.qubes.x86_64 then you can try kernel-latest-5.17.5-1.fc32.qubes.x86_64 from here:
https://yum.qubes-os.org/r4.1/current-testing/dom0/fc32/rpm/kernel-latest-5.17.5-1.fc32.qubes.x86_64.rpm